Related Experiment Video
Updated: Jun 19, 2026

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils
Published on: April 19, 2024
High oleic enhancement of palm olein via enzymatic interesterification
1Analytical and Quality Development Unit, Product Development and Advisory Services Division, Malaysian Palm Oil Board, Selangor, Malaysia. Siew@mpob.gov.my
Abstract:
Acidolysis to incorporate oleic acid into refined, bleached and deodorized (RBD) palm olein (IV 56) using various lipases (enzymes) as catalysts to increase the oleic content of the oil was investigated. Immobilised lipases (lipase PLG, Lipozyme TL IM, Lipozyme RM IM and Novozym 435) and non-immobilised lipase (lipase PL) were used in this study to compare the effectiveness of the selected lipases in catalyzing the reaction to produce a high oleic oil. The results showed that the TAG of OLO/OOL content was increased at least 4 fold and OOO content was increased at least 3 fold when a 5% enzyme load was used. Lipase PL showed the greatest increase in tri-unsaturated triacylglycerols (TAGs) content. A pilot scale experiment conducted using TL IM enzyme, followed by recovery of the oil and fractionation allows the production of oils with varying oleic contents. A high oleic content of 56% was achievable.
Related Concept Videos
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
Esters to Carboxylic Acids: Saponification
The reaction requires a base in stoichiometric amounts, which participates in the reaction and is not regenerated later. So, the base acts as a...
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids
Biofuels
Carboxylic Acids to Esters: Acid-Catalyzed (Fischer) Esterification Overview
Alkylation of β-Diester Enolates: Malonic Ester Synthesis

