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Updated: Jun 19, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Glycerol conversion to D-xylulose by a two-stage microbial reaction using Candida parapsilosis and Gluconobacter
Hiroshi Habe1, Tokuma Fukuoka, Dai Kitamoto
1Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
Abstract:
A yeast strain, 25N-2B, that produces D-arabitol from glycerol, was identified as Candida parapsilosis based on phylogenetic, morphological, physiological, and biochemical analyses. It produced 32.2 g/L D-arabitol from 170 g/L glycerol in a jar fermentor. The D-arabitol in the reaction mixture was then completely converted to D-xylulose using Gluconobacter oxydans NBRC3293. The product was isolated from the reaction mixture and confirmed to be D-xylulose by (1)H and (13)C-NMR and optical rotation.
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