Related Experiment Video
Updated: Jun 19, 2026
Transurethral Instillation Procedure in Adult Male Mouse
Published on: November 2, 2017
Chronic bacterial inflammation induces prostatic intraepithelial neoplasia in mouse prostate
J E Elkahwaji1, R J Hauke, C M Brawner
1Department of Internal Medicine, Section of Adult Oncology and Hematology and GU Oncology Research Laboratory, University of Nebraska Medical Center, Omaha, NE 68198-7680, USA. jelkahwaji@unmc.edu
Background:
Although the aetiology of prostate cancer remains unknown, we hypothesised that chronic bacterial insult has a major role in prostate carcinogenesis.
Methods:
Male C3H/HeOuJ mice, infected with phosphate-buffered saline or Escherichia coli bacteria, were killed at 5 days, or at 12 or 26 weeks. Harvested prostate tissues were evaluated for inflammatory responses and immunostained for neoplastic transformation markers.
Results:
All infected mice developed bacterial prostatitis. Control mice had no prostate infections or inflammation. Mice infected for 5 days showed foci of acute inflammation with infiltrating neutrophils and epithelial necrotic debris in the prostatic glandular lumen. All mice infected for 12 weeks had evidence of chronic inflammation with dense inflammatory infiltrates in the stroma. The prostatic epithelium showed varying degrees of atypical hyperplasia with increased epithelial cell layers and cytological atypia. At 26 weeks, the dysplastic changes were more pronounced and mimicked a prostatic intraepithelial neoplasia and high-grade dysplasia. Prostatic glands exhibiting reactive dysplasia had a stronger staining for oxidative DNA damage, increased epithelial cell proliferation, and a decrease in androgen receptor, GSTP1, p27(Kip1), and PTEN expression, when compared with control prostate glands.
Conclusion:
These data demonstrate that chronic inflammation induces focal prostatic glandular atypia and suggest a potential linkage between inflammation and prostatic neoplasia.
