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Updated: Jun 19, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Luis Miguel Sanchez-Brea1, Francisco Jose Torcal-Milla, Eusebio Bernabeu
1Optics Department, Applied Optics Complutense Group, Universidad Complutense de Madrid, Facultad de Ciencias Físicas, Ciudad Universitaria s.n., 28040 Madrid, Spain. sanchezbrea@fis.ucm.es
The Talbot effect can be canceled using a two-phase-grating mask, making devices more tolerant to distance variations. This configuration also quadruples fringe spatial frequency, enhancing device accuracy.
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