Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Live volumetric (4D) visualization and guidance of in vivo human ophthalmic surgery with intraoperative optical coherence tomography.

Scientific reports·2016
Same author

Comparison of optical coherence tomography imaging of cataracts with histopathology.

Journal of biomedical optics·2012
Same author

Optical Coherence Tomographic Imaging of Human Tissue at 1.55 μm and 1.81 μm Using Er- and Tm-Doped Fiber Sources.

Journal of biomedical optics·2012
Same author

Changes in the expression of the Alzheimer’s disease-associated presenilin gene in drosophila heart leads to cardiac dysfunction.

Current Alzheimer research·2011
Same author

Blood flow dynamics of one cardiac cycle and relationship to mechanotransduction and trabeculation during heart looping.

American journal of physiology. Heart and circulatory physiology·2011
Same author

Optical spatial tracking using coherent detection in the pupil plane.

Applied optics·2010
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Optical coherence microscopy in scattering media.

J A Izatt, M R Hee, G M Owen

    Optics Letters
    |October 22, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new optical coherence tomography technique improves optical sectioning in confocal microscopy. This method enables deeper imaging into scattering materials, outperforming single-backscatter theory predictions.

    More Related Videos

    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
    07:44

    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

    Published on: July 24, 2020

    Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
    07:51

    Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

    Published on: October 21, 2022

    Related Experiment Videos

    Last Updated: Jun 19, 2026

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
    07:44

    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

    Published on: July 24, 2020

    Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
    07:51

    Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

    Published on: October 21, 2022

    Area of Science:

    • Biomedical Optics
    • Microscopy Techniques
    • Optical Physics

    Background:

    • Confocal microscopy offers optical sectioning capabilities but struggles with imaging deep into scattering media.
    • Scattering in biological tissues and other materials limits penetration depth and image quality.
    • Existing techniques for deep imaging in scattering media have limitations.

    Purpose of the Study:

    • To introduce a novel technique combining optical coherence tomography (OCT) with confocal microscopy.
    • To enhance optical sectioning and enable deeper imaging into highly scattering media.
    • To validate the technique by comparing experimental results with theoretical predictions.

    Main Methods:

    • Development of a novel technique integrating optical coherence tomography principles into a confocal microscope setup.
    • Demonstration of enhanced optical sectioning capabilities.
    • Imaging experiments conducted in highly scattering media.
    • Comparison of imaging results with predictions from a single-backscatter theory.

    Main Results:

    • The novel technique successfully achieved enhanced optical sectioning in confocal microscopy.
    • Deep imaging into highly scattering media was demonstrated.
    • Experimental results showed good agreement with, and in some cases exceeded, the predictions of the single-backscatter theory.

    Conclusions:

    • The integration of optical coherence tomography offers a powerful approach for improving confocal microscopy.
    • This technique significantly enhances the ability to perform optical sectioning and deep imaging in scattering environments.
    • The findings suggest potential for advanced imaging applications in biological and material sciences.