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Updated: Jun 19, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
Published on: January 16, 2016
[Thermoregulation of catalytic activity of enzymes polymeric derivatives]
Abstract:
Trypsin was immobilized on polymeric carriers with low critical solution temperature (LCST). Homopolymer of N,N-diethylacrylamide (DEAA), random copolymers of DEAA and acrylamide (AA), and block copolymers polyDEAA-polyAA were used as the carriers. It was shown that at a temperature above LCST all carriers have a conformation change and trypsin's polymeric derivatives precipitate. The maximal activity after phase transition keeps trypsin, immobilized on polyDEAA block in polyDEAA-polyAA block-copolymer.
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