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Updated: Jun 19, 2026

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Carbon and nitrogen source effects on basidiomycetes exopolysaccharide production
V I Elisashvili1, E T Kachlishvili, S P Wasser
1Durmishidze Institute of Biochemistry and Biotechnology, 10 km Agmashenebeli alley, 0159 Tbilisi, Georgia. velisashvili@hotmail.com
Abstract:
The capability to synthesize the extracellular polysaccharide (EPS) is widespread among eight mushroom species which accumulated 0.6-2.2 g/l of EPS in submerged cultivation. Glucose, maltose, and mannitol were the most appropriate carbon sources for biomass and EPS production. Organic nitrogen sources appeared to be the most suitable nitrogen sources for biomass and EPS accumulation. The cultivation process in shake flasks was successfully reproduced in a laboratory fermentor with enhanced EPS production. The highest yield of EPS (3.8-4.0 g/l) was achieved in cultivation of Agaricus nevoi and Inonotus levis.
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