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Updated: Jun 19, 2026
06:58
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Carbon and nitrogen source effects on basidiomycetes exopolysaccharide production
V I Elisashvili1, E T Kachlishvili, S P Wasser
1Durmishidze Institute of Biochemistry and Biotechnology, 10 km Agmashenebeli alley, 0159 Tbilisi, Georgia. velisashvili@hotmail.com
Prikladnaia Biokhimiia I Mikrobiologiia
|October 23, 2009
Summary
Eight mushroom species can produce extracellular polysaccharide (EPS) in submerged cultivation. Optimal production of mushroom EPS utilized specific carbon and organic nitrogen sources, with highest yields from Agaricus nevoi and Inonotus levis.
Area of Science:
- Mycology
- Biotechnology
- Biochemistry
Background:
- Extracellular polysaccharides (EPS) are microbial biopolymers with diverse applications.
- Mushroom cultivation offers a potential source for novel EPS production.
Purpose of the Study:
- To investigate the capacity of eight mushroom species for EPS synthesis via submerged cultivation.
- To identify optimal carbon and nitrogen sources for enhancing mushroom EPS production.
- To scale up EPS production from shake flasks to laboratory fermentors.
Main Methods:
- Submerged cultivation of eight mushroom species.
- Screening of various carbon sources (glucose, maltose, mannitol) and nitrogen sources (organic).
- Biomass and EPS yield quantification.
- Fermentor-based cultivation for process optimization.
Main Results:
- All eight mushroom species demonstrated EPS synthesis capabilities, accumulating 0.6-2.2 g/l.
- Glucose, maltose, and mannitol were identified as superior carbon sources.
- Organic nitrogen sources supported higher biomass and EPS accumulation.
- Fermentor cultivation improved EPS yields, reaching 3.8-4.0 g/l for Agaricus nevoi and Inonotus levis.
Conclusions:
- Mushroom submerged cultivation is a viable method for EPS production.
- Specific carbon and organic nitrogen sources significantly enhance EPS yields.
- Agaricus nevoi and Inonotus levis show high potential for industrial-scale EPS production.
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