Related Experiment Video
Updated: Jun 19, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
Polymorphisms in tumor necrosis factor-A gene and prostate cancer risk in North Indian cohort
Pravin Kesarwani1, Anil Mandhani, Rama Devi Mittal
1Department of Urology and Renal Transplantation, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Purpose:
The proinflammatory cytokine tumor necrosis factor-alpha has an important role in the pathogenesis of prostate cancer. Single nucleotide polymorphisms in the promoter region of the TNF-A gene alter tumor necrosis factor-alpha transcription. Thus, we studied the association of 4 SNPs in the promoter region of TNF-A gene, including -1031T>C, -863C>A, -857 C>T and -308 G>A, in a North Indian cohort of patients with prostate cancer.
Materials And Methods:
The study involved 453 subjects. All 197 case and 256 control samples were genotyped for the 4 promoter polymorphisms in the TNF-A gene using allele specific polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism.
Results:
Results showed an increased risk of prostate cancer for the TNF-A -1031 CC genotype (OR 2.01, p = 0.03). No significant association was observed for the TNF-A -863 C>A, TNF-A -857 C>T or -308 G>A polymorphisms. Haplotype analysis revealed that TNF-A -1031C-863C-857T-308G was significantly associated with prostate cancer risk (OR 2.22, p = 0.013). Moreover, the TNF-A -1031 C and -857 T alleles were associated with higher tumor grade and an increased risk of tumor progression and metastasis.
Conclusions:
These results show that TNF-A polymorphisms have an important role in prostate cancer pathogenesis. Results are in line with findings in other studies from the West and to our knowledge for the first time from India indicating the involvement of immune system genes in prostate cancer pathogenesis.
Related Concept Videos
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase
Single Nucleotide Polymorphisms-SNPs
Cancer-Critical Genes I: Proto-oncogenes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
