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Subtyping of emm1 group A Streptococci causing invasive infections in France

P Bidet1, E Lesteven, C Doit

  • 1Laboratoire d'Etudes de Génétique Bactérienne dans les Infections de l'Enfant, Université Paris VII, UFR Médicale, EA 3105, Assistance Publique-Hôpitaux de Paris, Paris, France.

Journal of Clinical Microbiology
|October 23, 2009
PubMed

Insights

Researchers identified 24 distinct genetic types of M/emm1 group A Streptococcus bacteria in France. This study analyzed strains from both infected individuals and control cases.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Group A Streptococcus (GAS) is a significant human pathogen.
  • The M/emm1 genotype is a common and often invasive type of GAS.

Purpose of the Study:

  • To genetically characterize M/emm1 group A Streptococcus strains.
  • To investigate the genetic diversity of M/emm1 GAS in France.

Main Methods:

  • Polymerase Chain Reaction (PCR) amplification of toxin-encoding genes.
  • Sequencing of the sic gene for genotyping.

Main Results:

  • 24 distinct genotypes were identified among 47 M/emm1 GAS isolates.
  • Strains were isolated from children and adults in France.
  • Infections included 9 cases with four clusters and 38 unrelated invasive cases.

Conclusions:

  • Significant genetic diversity exists within the M/emm1 GAS population in France.
  • Genotyping methods can effectively differentiate GAS strains.

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