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Related Experiment Video

Updated: Jun 19, 2026

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Published on: December 12, 2017

Integrase-defective lentiviral vectors: progress and applications.

M B Banasik¹, P B McCray

¹Department of Pediatrics, Program in Gene Therapy, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.

|October 23, 2009

Summary

Non-integrating lentiviral vectors (LVs) provide transient gene expression, avoiding risks of insertional mutagenesis associated with integrating LVs. This technology offers new therapeutic and research avenues for gene delivery.

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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

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Published on: May 24, 2012

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Last Updated: Jun 19, 2026

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

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An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

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Published on: January 12, 2018

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Area of Science:

Gene therapy
Molecular biology
Virology

Background:

Lentiviral vectors (LVs) are widely used for gene delivery due to their large packaging capacity and ability to achieve long-term transgene expression.
However, the integration of LV gene cassettes into the host genome carries a risk of insertional mutagenesis, potentially disrupting gene expression.
This integration is not always necessary for therapeutic or research applications, creating a need for alternative vector systems.

Purpose of the Study:

To discuss the development and biological characterization of non-integrating lentiviral vectors (LVs).
To explore the potential applications of integration-defective LVs where transient gene expression is desired.
To highlight new directions for the advancement of non-integrating LV technology.

Main Methods:

Review of recent publications detailing the development of integration-defective LVs.
Analysis of biological characterization data for these novel vectors.
Discussion of potential applications based on vector properties.

Main Results:

Non-integrating LVs have been successfully developed and characterized.
These vectors offer transient gene expression, mitigating risks associated with genomic integration.
Pseudotyping allows for broad or specific cell tropism, enhancing versatility.

Conclusions:

Integration-defective LVs represent a valuable tool for applications requiring transient gene expression.
They offer a safer alternative to integrating LVs by avoiding insertional mutagenesis.
Further development holds promise for expanded therapeutic and research applications in gene delivery.