Related Experiment Video
Updated: Jun 19, 2026

Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies
Published on: April 12, 2022
Determination of aliphatic and aromatic aldehydes in polluted airs as their 2,4-dinitrophenylhydrazones by high
K Kuwata1, M Uebori, Y Yamasaki
1Environmental Pollution Control Center, Osaka, 63 Ban, 1 Chome, Nakamichi, Higashinari-ku, Osaka City 537, Japan.
Abstract:
A reversed-phase high performance liquid chromatography system with a LiChrosorb RP-18 column and acetonitrile-water mobile phase has been applied to the determination of C1-C6 aliphatic and aromatic aldehydes in polluted airs such as urban air, industrial emission, auto exhaust, and tobacco smoke. The aldehydes in a sample were allowed to react with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazone derivatives. The derivatives were extracted with chloroform and concentrated. The residue in acetonitrile was analyzed by high performance liquid chromatography. The derivatives of C1-C6 aliphatic aldehydes could be separated from the derivatives of the same molecular weight ketones, and aromatic aldehydes such as benzaldehyde, and isomers of tolualdehydes could be separated without effects of aliphatic carbonyl compounds. The aldehydes in the low ppm or ppb levels in air samples could be determined with good accuracy.
More Related Videos
07:32Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
11:44Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
Published on: March 6, 2016
Related Concept Videos
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
Gas Chromatography: Types of Detectors-II
High-Performance Liquid Chromatography: Types of Detectors