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Related Experiment Videos

Human thymic epithelial cells produce interleukin-3.

A H Dalloul1, M Arock, C Fourcade

  • 1Cellular Immunology Department, CNRS URA 186, La Pitié-Salpêtrière Hospital, Paris, France.

Blood
|January 1, 1991
PubMed
Summary

Human thymic epithelial cells produce Interleukin-3 (IL-3), a hematopoietic growth factor. This finding challenges the prior belief that only activated T lymphocytes produce IL-3.

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Interleukin-3 (IL-3) is a known hematopoietic growth factor.
  • IL-3 is typically associated with activated T lymphocytes.
  • Human thymic stroma supernatants promote myeloid stem cell proliferation.

Purpose of the Study:

  • To investigate if IL-3 produced by human thymic epithelial cells (TEC) accounts for the myeloid stem cell proliferation observed.
  • To determine the source of IL-3 production within the human thymus.

Main Methods:

  • Isolation of human thymic epithelial cells (TEC), fibroblasts, and adherent cells.
  • Assay of culture supernatants for myeloid colony-promoting activity.
  • Use of anti-IL-3 monoclonal antibody to assess IL-3's role.

Related Experiment Videos

  • In situ hybridization to detect IL-3 mRNA in TEC.

    • Main Results:

    • Supernatants from TEC, but not fibroblasts or adherent cells, supported myeloid colony-forming unit growth.
    • Addition of anti-IL-3 antibody reduced the colony-promoting activity of TEC supernatants.
    • In situ hybridization confirmed the presence of IL-3 mRNA within TEC.
    • TEC culture conditions influenced IL-3 production.

    Conclusions:

    • Human thymic epithelial cells (TEC) are a source of Interleukin-3 (IL-3).
    • IL-3 production is not exclusive to activated T lymphocytes.
    • TEC contribute to the hematopoietic growth factor environment within the thymus.