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Related Experiment Video

Updated: Jun 19, 2026

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Published on: February 2, 2015

Histamine stimulates human lung fibroblast migration.

Tadashi Kohyama¹, Yasuhiro Yamauchi, Hajime Takizawa

¹Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. koyama-tky@umin.ac.jp

Molecular and Cellular Biochemistry

|October 24, 2009

Summary

Histamine stimulates human lung fibroblast migration, potentially impacting wound healing and lung fibrosis. This effect is mediated by the H4 receptor, as shown by inhibition with a specific antagonist.

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Published on: June 29, 2021

Area of Science:

Immunology and Cell Biology
Pulmonary Medicine

Background:

Histamine is a key mediator in allergic inflammation, released by mast cells and basophils.
Fibroblast migration is crucial for tissue repair and can contribute to fibrotic diseases.

Purpose of the Study:

To investigate the effect of histamine on the in vitro migration of human fetal lung fibroblasts (HFL-1).
To determine the role of histamine and its receptor in fibroblast chemotaxis towards human plasma fibronectin (HFn).

Main Methods:

Utilized the blind-well chamber technique to assess HFL-1 cell migration.
Tested histamine alone and in combination with HFn, a known chemoattractant.
Investigated the involvement of the H4 receptor using a specific antagonist (JNJ7777120) and pertussis toxin.

Main Results:

Histamine alone did not induce chemotaxis but significantly augmented HFn-induced HFL-1 migration (up to 290.6%).
The stimulatory effect exhibited a bell-shaped concentration-response curve and increased with time.
Inhibition by an H4 receptor antagonist and pertussis toxin confirmed H4 receptor mediation.

Conclusions:

Histamine stimulates human lung fibroblast migration, suggesting a role in regulating wound healing.
This histamine-induced fibroblast migration may contribute to the pathogenesis of lung fibrotic disorders.
The H4 receptor is implicated in mediating histamine's effects on lung fibroblasts.