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[Nandeshi: a powerful inhibitor of human acrosin activity]
Gui-Hong Song1, Jue Zhang, Xiao-Meng Zhang
1Research Center of Reproductive Medicine, Changhai Hospital, 2. School of Pharmacology, The Second Military, Shanghai 200433, China.
Objective:
To evaluate the inhibitory effect of Nandeshi, an acrosin inhibitor, on human acrosin activity.
Methods:
We collected sperm samples from 10 healthy fertile men and cultured them with Nandeshi at 30 degrees C for 5 minutes at the concentrations of 0. 100, 0.120, 0.144, 0.173, 0.207, 0.249, 0.299, 0.358 and 0.430 mmol/L, with the controls treated with a well-known acrosin inhibitor N-alpha-p-tosyl-L-lysine chloromethylketone (TLCK) at 150.0, 189.8, 213.6, 240.3, 270.3, 304.1 and 342.1 mmol/L. Then we determined the residual activity of human acrosin by improved Kennedy assay.
Results:
The residual activity of acrosin was negatively correlated with the Nandeshi concentration, and Nandeshi exhibited an inhibition rate about 800 times that of TLCK.
Conclusion:
Nandeshi has a powerful inhibitory effect on human acrosin, and improved Kennedy assay is a simple, practical and highly sensitive technique for the detection of human acrosin activity.
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