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Updated: Jun 19, 2026

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
Published on: October 7, 2020
[Research progress in the in vitro culture of testicular spermatogenic cells]
Juan Gu1, Yi-Bo Wang, Cong-Hui Han
1Southeast University Institute of Reproductive Medicine/Center of Reproductive Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, China.
Abstract:
The production of sperm is a very complicated and orderly process, which involves the proliferation and differentiation of cells. The in vitro culture of spermatogenic cells includes tissue culture and cell culture. This study focuses on the advances in the in vitro culture of spermatogenic cells, testicular tissue culture and their culture conditions, and sums up the advantages and disadvantages of various culture methods, in an attempt to find the best method for differentiating spermatogenic cells in vitro and make the culture of spermatogenic cells an important means of treating male infertility.
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