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Updated: Jun 19, 2026
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
Published on: February 9, 2017
PRDM16 expression in the developing mouse embryo
Kristin H Horn1, Dennis R Warner, Michele Pisano
1University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, School of Dentistry, 501 South Preston Street, Louisville, KY 40292, USA.
Abstract:
PRDM16 is a member of the PR domain-containing protein family and is associated with various disease states including myelodysplastic syndrome and adult T-cell leukemia, as well as developmental abnormalities such as cleft palate. It is also known to act as a regulator of cell differentiation. Expression analysis of PRDM16 is limited, especially within the developing embryo. The current study evaluated the temporal and spatial localization of PRDM16 during early mouse development (embryonic days 8.5-14.5). PRDM16 was first detected on E9.5 in a limited number of tissues and by E14.5, was expressed in a broad range of developing tissues including those of the brain, lung, kidney, and gastrointestinal tract. The expression pattern is consistent with a role for PRDM16 in the development of multiple tissues. Collectively, these studies are the first to characterize the expression of the PRDM16 gene during early murine development.
Insights
This study maps the expression of PRDM16 during early mouse development. PRDM16 is found in various developing tissues, suggesting a broad role in embryonic development.
Area of Science:
- Developmental biology
- Gene expression analysis
- Molecular biology
Background:
- PRDM16 is a member of the PR domain-containing protein family.
- It is implicated in diseases like myelodysplastic syndrome and adult T-cell leukemia.
- PRDM16 also functions as a regulator of cell differentiation.
Purpose of the Study:
- To investigate the temporal and spatial expression patterns of PRDM16 during early mouse embryogenesis.
- To provide a comprehensive understanding of PRDM16 localization in developing tissues.
Main Methods:
- Utilized whole-mount in situ hybridization and immunohistochemistry.
- Examined PRDM16 expression in mouse embryos from embryonic day 8.5 to 14.5.
Main Results:
- PRDM16 expression was first detected at embryonic day 9.5 in specific tissues.
- By embryonic day 14.5, PRDM16 showed widespread expression across numerous developing organs, including brain, lung, kidney, and gastrointestinal tract.
- The observed expression patterns suggest PRDM16's involvement in the development of multiple organ systems.
Conclusions:
- This study presents the first detailed characterization of PRDM16 gene expression during early murine development.
- The findings support a significant role for PRDM16 in the development and differentiation of various embryonic tissues.
