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Updated: Jun 19, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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Published on: December 30, 2016

Conversion of peripheral CD4(+)CD25(-) T cells to CD4(+)CD25(+) regulatory T cells by IFN-gamma in patients with

Shuo Huang1, Lei Li, Songlan Liang

  • 1Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.

Journal of Neuroimmunology
|October 27, 2009
PubMed

CD4(+)CD25(-) T cells from 25 Guillain-Barré syndrome (GBS) patients were stimulated by IFN-gamma to generate the induced CD4(+)CD25(+) regulatory T cells (iTregs). Then, their phenotypical and functional characterizations were analyzed. Results show that iTregs can be generated from CD4(+)CD25(-) T cells in GBS patients by IFN-gamma in the presence of activating anti-CD3 and anti-CD28 antibodies in vitro. When CD4(+)CD25(-) T cells are stimulated by 40ng/ml IFN-gamma for 3days, the ratio of conversion is the highest under this condition and iTregs are similar to naturally occurring CD4(+)CD25(+) regulatory T cells of healthy donors phenotypically and functionally.

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