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Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
Published on: July 3, 2016
Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment
Soo-Young Lee1, Tae-Kwang Oh1, Jung-Hoon Yoon1
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Abstract:
A Gram-staining-positive, endospore-forming, motile, rod-shaped bacterium, strain AD-1(T), was isolated from a tidal flat sediment of the coast of Hwangdo on the Yellow Sea, Korea. Strain AD-1(T) grew optimally at pH 7.0-7.5 and 40 degrees C and in the presence of 5-10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD-1(T) was most closely related to Thalassobacillus devorans G-19.1(T) (98.0 % sequence similarity) and Thalassobacillus cyri HS286(T) (97.8 %). The cell-wall peptidoglycan was based on meso-diaminopimelic acid and MK-7 was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The major fatty acids (>10 % of total fatty acids) were iso-C(15 : 0), iso-C(17 : 0) and anteiso-C(15 : 0). The DNA G+C content of strain AD-1(T) was 45.2 mol%. It appears reasonable to classify strain AD-1(T) as a member of the genus Thalassobacillus. There were differences in fatty acid profiles and phenotypic and genetic characteristics between strain AD-1(T) and the type strains of the two Thalassobacillus species. On the basis of the data presented, strain AD-1(T) represents a novel species within the genus Thalassobacillus, for which the name Thalassobacillus hwangdonensis sp. nov. is proposed. The type strain is AD-1(T) (=KCTC 13254(T) =CCUG 56607(T)).
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