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Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Complete genome sequence of Anaplasma marginale subsp. centrale.

David R Herndon1, Guy H Palmer, Varda Shkap

  • 1Animal Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164, USA.

Journal of Bacteriology
|October 27, 2009
PubMed
Summary

Anaplasma marginale subsp. centrale, a century-old vaccine, was sequenced and compared to virulent strains. This research identifies key outer membrane proteins for developing safer inactivated vaccines against anaplasmosis.

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Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Immunology

Background:

  • Anaplasma marginale is a tick-borne pathogen causing significant economic losses in cattle.
  • Anaplasma marginale subsp. centrale has been utilized as a live attenuated vaccine for approximately 100 years.
  • Understanding the genetic differences between attenuated and virulent strains is crucial for vaccine improvement.

Purpose of the Study:

  • To sequence the genome of Anaplasma marginale subsp. centrale.
  • To compare the genome of the attenuated strain with virulent Anaplasma marginale senso stricto strains.
  • To identify potential outer membrane protein targets for developing safer inactivated vaccines.

Main Methods:

  • Whole-genome sequencing of Anaplasma marginale subsp. centrale.
  • Comparative genomic analysis with publicly available Anaplasma marginale senso stricto genomes.
  • Bioinformatic analysis to identify differences in outer membrane protein genes.

Main Results:

  • The genome of Anaplasma marginale subsp. centrale was successfully sequenced.
  • Comparative analysis revealed specific genetic variations, particularly in outer membrane protein genes, between the attenuated and virulent strains.
  • This comparison significantly reduced the number of candidate outer membrane proteins for vaccine development.

Conclusions:

  • The genomic comparison provides valuable insights into the genetic basis of attenuation in Anaplasma marginale subsp. centrale.
  • Identified outer membrane proteins represent promising targets for the development of novel, safer inactivated vaccines against anaplasmosis.
  • This study enhances our understanding of the genetic diversity within Anaplasma marginale senso lato.