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Updated: Jun 19, 2026
Murine Prostate Micro-dissection and Surgical Castration
Published on: May 11, 2016
miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice
Cyrielle Clapé1, Vanessa Fritz, Corinne Henriquet
1Institut de Recherche en Cancérologie de Montpellier, Montpellier, France.
Background:
Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo.
Results:
Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer.
Conclusions:
miR-143 is as a new target for prostate cancer treatment.
