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Updated: Jun 19, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Rapid changes in rotaviral genotypes in Ecuador.

Maria Eloisa Hasing¹, Gabriel Trueba, Maria Ines Baquero

¹Institute of Microbiology, Universidad San Francisco de Quito, Quito, Ecuador.

Journal of Medical Virology

|October 27, 2009

Summary

Rotavirus genotype G9, once dominant in Ecuador, was rapidly replaced by G1 and G2 strains between 2005 and 2008. This shift highlights the need for ongoing rotavirus genotype surveillance to guide effective vaccination strategies.

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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

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Area of Science:

Virology
Epidemiology
Public Health

Background:

The G9P[8] rotavirus genotype was prevalent in Ecuador in 2005.
Rotavirus remains a significant cause of diarrheal disease globally.

Purpose of the Study:

To conduct a temporal analysis of rotavirus genotype distribution in Ecuador from 2005 to early 2008.
To investigate the replacement dynamics of rotavirus genotypes in rural and urban settings.

Main Methods:

Collection of 3,300 stool samples (cases and controls) in rural Ecuador (2003-2008).
Collection of 59 rotavirus-positive fecal samples from children in urban Quito (2005-2007).
RT-PCR analysis for rotavirus genotype determination.

Main Results:

Rotavirus genotype G9 prevalence decreased significantly from 79% to 9% in rural areas and 79% to 37% in urban Quito between 2005 and 2007.
Rotavirus genotype G2 prevalence increased substantially, from 0% to 43% in rural areas and 3% to 57% in urban Quito during the same period.
Genotypes G1 and G2 replaced G9 as the dominant strains in both study locations.

Conclusions:

The rapid replacement of rotavirus genotype G9 by G1 and G2 necessitates continuous surveillance.
Monitoring rotavirus genotype shifts is crucial for informing and adapting rotavirus vaccination programs in Ecuador.