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Updated: Jun 19, 2026

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies
Published on: June 1, 2019
[Morphological comparison of two species of Theileria in sheep]
You-Quan Li1, Gui-Quan Guan, Mi-Ling Ma
1State Key Laboratory of Veterinary Pathogen Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Abstract:
Two splenectomized sheep were infected respectively with Theileria luwenshuni and T. uilenbergi, 2 species identified by PCR. When piroplasms were found in blood smears from ears of the sheep, morphological observation on the Theileria spp. was carried out by optical microscopy. By Giemsa staining, the cytoplasm exhibited in slight blue and nucleus in purple. The two Theileria species displayed various shapes, but pyriform-shaped, round-shaped and needle-shaped parasites appeared in every stage of the infection. In fact, there is no significant morphological difference between the two species.
