Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An AQP1 allele associated with co(a-b-) phenotype.

Immunohematology·2013
Same author

The molecular basis of the LU:7 and LU:-7 phenotypes.

Immunohematology·2013
Same author

Possible suppression of fetal erythropoiesis by the Kell blood group antibody anti-Kp(a).

Immunohematology·2012
Same author

Molecular basis of the rare gene complex, DIVa(C)-, which encodes four low-prevalence antigens in the Rh blood group system.

Vox sanguinis·2011
Same author

Red cells from the original JAL+ proband are also DAK+ and STEM+.

Vox sanguinis·2011
Same author

The low prevalence Rh antigen Be(a) (Rh36) is associated with RHCE*ce 662C>G in exon 5, which is predicted to encode Rhce 221Arg.

Vox sanguinis·2009

Related Experiment Video

Updated: Jun 19, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Published on: August 6, 2011

Principles of PCR-based assays.

K Hue-Roye1, S Vege

  • 1New York Blood Center, New York, NY 10065, USA.

Immunohematology
|October 28, 2009
PubMed
Summary

DNA-based blood group testing is becoming popular. This review covers Polymerase Chain Reaction (PCR) assays and microarray platforms used in transfusion medicine for accurate blood group prediction.

Area of Science:

  • Molecular Biology
  • Genetics
  • Transfusion Medicine

Background:

  • Blood group determination is crucial for safe transfusions.
  • DNA-based assays offer precise and reliable blood group prediction.
  • Advancements in molecular techniques enhance diagnostic capabilities.

Purpose of the Study:

  • To review the principles of Polymerase Chain Reaction (PCR)-based assays.
  • To highlight the application of DNA analysis in transfusion medicine.
  • To discuss emerging molecular testing platforms.

Main Methods:

  • DNA extraction from various sources.
  • Polymerase Chain Reaction (PCR) amplification for target DNA.
  • Analysis using standard PCR with RFLP/sequencing, allele-specific PCR, multiplex PCR, and real-time PCR.

More Related Videos

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Related Experiment Videos

Last Updated: Jun 19, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Published on: August 6, 2011

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

  • Microarray platforms for high-throughput nucleotide analysis.

    • Main Results:

    • PCR-based assays are effective for blood group genotyping.
    • Different PCR techniques offer varying levels of specificity and throughput.
    • Microarray platforms enable simultaneous analysis of multiple genetic markers.
    • These molecular methods provide accurate blood group prediction.

    Conclusions:

    • DNA-based assays are powerful tools for blood group determination.
    • PCR and microarray technologies are increasingly utilized in transfusion medicine.
    • These methods enhance the safety and efficiency of blood transfusion practices.