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Related Experiment Video

Updated: Jun 19, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

Antimicrobial susceptibility testing: a primer for clinicians.

Kristi M Kuper¹, Deborah M Boles, John F Mohr

¹Department of Clinical Affairs, Cardinal Health, Houston, TX 77077, USA. kristine.kuper@cardinalhealth.com

Pharmacotherapy

|October 28, 2009

Summary

Understanding antimicrobial susceptibility testing (AST) methods is crucial for appropriate antibiotic selection. Nuances in AST techniques can impact results, influencing patient care and antibiotic stewardship programs.

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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

Area of Science:

Clinical Microbiology
Infectious Diseases
Pharmacology

Background:

Appropriate antimicrobial use remains a significant challenge in healthcare settings.
Antimicrobial susceptibility testing (AST) provides critical data for guiding effective antibiotic therapy.
Misinterpretation of AST results due to methodological nuances can lead to suboptimal antibiotic choices.

Purpose of the Study:

To describe common antimicrobial susceptibility testing methods used in clinical microbiology laboratories.
To review how variations in testing techniques can affect reported susceptibility results.
To enhance clinician understanding of AST for improved patient outcomes and antibiotic stewardship.

Main Methods:

Review of common qualitative and quantitative antimicrobial susceptibility testing methods.
Discussion of automated susceptibility testing systems.
Overview of screening and confirmatory tests for antibiotic-resistant organisms.

Main Results:

Subtle differences in AST methods and execution can significantly influence test outcomes.
A thorough understanding of these nuances is essential for accurate interpretation of susceptibility data.
Variations in testing can lead to misinterpretation and inappropriate antibiotic selection.

Conclusions:

Clinicians with a strong grasp of AST principles can better interpret results and optimize antibiotic therapy.
Improved understanding of AST strengthens institutional antibiotic stewardship programs.
Accurate AST interpretation directly contributes to improved patient outcomes and combats antimicrobial resistance.