Related Experiment Video
Updated: Jun 19, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as
1Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China. lyzhang26@yahoo.com.cn
Abstract:
A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM NDA, 20 mM borate buffer (pH 9.2) with the reaction time of 4 min at room temperature. The capillary electrophoresis analysis of GSH could be achieved in less than 120 s using 10 mM sodium tetraborate (pH 9.2) containing 2.5 mM beta-cyclodextrin (beta-CD) as the running buffer, and the detection limit of 2.5 x 10(-9) M (S/N=3) was obtained. This method was successfully applied to analyze the content of GSH in tobacco BY-2 cells.
Related Concept Videos
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation

