Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visna virus RNA synthesis.

M Brahic, P Filippi, R Vigne

    Journal of Virology
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Visna virus, a retrovirus, persists by restricting its genetic information expression. This study quantifies viral RNA synthesis in permissive cells, establishing a baseline for understanding visna virus restriction in vivo.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of pectoral muscle atrophy in critically ill patients.

    Medicina intensiva·2022
    Same author

    Autotransplatation of immature third molars as substitutes for congenitally missing second premolars: an alternative solution in a young patient with oligodontia.

    Journal of biological regulators and homeostatic agents·2020
    Same author

    Vaccine-Associated Maintenance of Epithelial Integrity Correlated With Protection Against Virus Entry.

    The Journal of infectious diseases·2018
    Same author

    Reconstruction of primary vertices at the ATLAS experiment in Run 1 proton-proton collisions at the LHC.

    The European physical journal. C, Particles and fields·2017
    Same author

    Topological cell clustering in the ATLAS calorimeters and its performance in LHC Run 1.

    The European physical journal. C, Particles and fields·2017
    Same author

    Vaccine-modified NF-kB and GR signaling in cervicovaginal epithelium correlates with protection.

    Mucosal immunology·2017
    Same journal

    Magnolol inhibits porcine epidemic diarrhea virus infection by suppressing cathepsin L expression <i>in vitro</i> and <i>in vivo</i>.

    Journal of virology·2026
    Same journal

    Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

    Journal of virology·2026
    Same journal

    Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

    Journal of virology·2026
    Same journal

    MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

    Journal of virology·2026
    Same journal

    Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

    Journal of virology·2026
    Same journal

    A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

    Journal of virology·2026
    See all related articles

    Area of Science:

    • Virology
    • Molecular Biology
    • Immunology

    Background:

    • Visna virus, a retrovirus, causes slow infections characterized by viral persistence despite host immune response.
    • Viral genetic information expression restriction contributes to visna virus persistence and slow spread.
    • The precise interruption point and mechanism of this restriction in the visna virus life cycle remain unknown.

    Purpose of the Study:

    • To initiate a molecular analysis of visna virus restriction, focusing on transcriptional regulation.
    • To establish baseline levels of viral RNA synthesis under permissive conditions for future in vivo studies.

    Main Methods:

    • Quantification of viral RNA synthesis in infected cells.
    • Analysis of RNA localization (nucleus vs. cytoplasm) and transport.

    Related Experiment Videos

  • Sedimentation analysis of viral RNA to determine size classes.

    • Main Results:

    • Uninfected cells lack visna virus-related RNA sequences.
    • Parental RNA rapidly translocates to the nucleus; viral RNA is synthesized there and exported to the cytoplasm.
    • RNA synthesis is largely exponential, reaching approximately 4,000 copies/cell.
    • Both nuclear and cytoplasmic viral RNA exist in 35S and 10-20S size classes.
    • Viral messenger RNA shares polarity with genome RNA and exhibits similar size distributions.

    Conclusions:

    • This study quantifies visna virus RNA synthesis, providing essential baseline data.
    • The findings elucidate key aspects of visna virus transcription and RNA processing.
    • Understanding these molecular mechanisms is crucial for investigating visna virus persistence in vivo.