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Related Experiment Video

Updated: Jun 19, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

Platelet adhesion and aggregation under flow using microfluidic flow cells.

Carolyn G Conant1, Michael A Schwartz, Tanner Nevill

  • 1Fluxion Biosciences, Inc. carolyn.conant@fluxionbio.com

Journal of Visualized Experiments : Jove
|October 28, 2009
PubMed
Summary

This study introduces a microfluidic assay for platelet aggregation that mimics blood flow. This method accurately assesses platelet function and drug responses under physiological conditions.

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Microfluidics

Background:

  • Platelet aggregation is crucial for hemostasis following vascular injury.
  • Conventional assays do not replicate the physiological shear flow conditions present in blood vessels.
  • Understanding platelet behavior under shear flow is vital for thrombosis research and drug development.

Purpose of the Study:

  • To develop and validate a microfluidic assay for studying platelet aggregation under physiological shear flow.
  • To provide a more accurate platform for assessing platelet function compared to static assays.
  • To enable the evaluation of platelet aggregation inhibitors.

Main Methods:

  • A microfluidic well-plate system was engineered to simulate vascular injury.

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Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

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Last Updated: Jun 19, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow
11:42

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Published on: July 10, 2017

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
08:50

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

  • Extracellular matrix proteins (collagen I, von Willebrand factor) were immobilized within microfluidic channels.
  • Whole blood, labeled with fluorescent dye, was perfused through the channels at controlled flow rates.
  • Inhibitors were introduced to generate dose-response data (IC50 values).

    • Main Results:

    • The microfluidic assay successfully induced platelet activation and aggregation under controlled shear flow.
    • The system allowed for the quantitative assessment of platelet aggregation.
    • Dose-response curves for platelet aggregation inhibitors were generated, demonstrating assay sensitivity.

    Conclusions:

    • The developed microfluidic assay effectively replicates physiological shear flow conditions for studying platelet aggregation.
    • This platform offers a more accurate and physiologically relevant method for platelet function analysis.
    • The assay is suitable for screening anti-platelet therapies and understanding thrombosis mechanisms.