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Related Experiment Video

Updated: Jun 19, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Real-time resonant holography using bacteriorhodopsin thin films.

J E Millerd, N J Brock, M S Brown

    Optics Letters
    |October 28, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a novel holographic technique for real-time, 2D species concentration measurement in turbulent flow. The method uses dual-wavelength holography to visualize sodium vapor dynamics with high sensitivity.

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    Last Updated: Jun 19, 2026

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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    Published on: February 8, 2014

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
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    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

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    Area of Science:

    • Fluid dynamics
    • Optical measurement techniques
    • Spectroscopy

    Background:

    • Turbulent flows present challenges for real-time species concentration measurement.
    • Holography offers potential for non-intrusive, quantitative flow field analysis.
    • Bacteriorhodopsin polymer films are explored for holographic recording media.

    Purpose of the Study:

    • To develop and demonstrate a real-time, two-dimensional (2D) measurement technique for species concentration in turbulent flows.
    • To characterize the holographic recording and decay dynamics in a bacteriorhodopsin-doped polymer film.
    • To achieve species-specific visualization of sodium vapor using holographic interferometry.

    Main Methods:

    • Pulsed holographic recording and decay dynamics were characterized for a 46-micrometer polymer film containing bacteriorhodopsin.
    • Species-specific holographic interferograms of sodium vapor were produced by simultaneously recording two holograms at different wavelengths.
    • One hologram was tuned near a sodium absorption feature, while the other was tuned off resonance.

    Main Results:

    • Demonstrated real-time, 2D mapping of species concentration within a turbulent flow field.
    • Characterized the performance of bacteriorhodopsin polymer films for holographic applications.
    • Successfully generated species-specific holographic interferograms for sodium vapor visualization.

    Conclusions:

    • The developed holographic technique enables effective real-time, 2D species concentration measurement in turbulent flows.
    • The study provides insights into the dynamics of holographic recording in bacteriorhodopsin-doped films.
    • The dual-wavelength holographic approach offers high sensitivity and specificity for species visualization.