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Updated: Jun 19, 2026

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics
Published on: March 18, 2021
Backstep scanning ion conductance microscopy as a tool for long term investigation of single living cells
Patrick Happel1, Irmgard D Dietzel
1Department of Molecular Neurobiochemistry, Ruhr-University Bochum, D-44870 Bochum, Germany. patrick.happel@rub.de.
Abstract:
Scanning ion conductance microscopy (SICM) is a suitable tool for imaging surfaces of living cells in a contact-free manner. We have shown previously that SICM in backstep mode allows one to trace the outlines of entire cell somata and to detect changes in cellular shape and volume. Here we report that SICM can be employed to quantitatively observe cellular structures such as cell processes of living cells as well as cell somata of motile cells in the range of hours.

