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Updated: Jun 19, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
[Phylogenetic analysis and PCR-restriction fragment length polymophism identification of Salmonella based on grOEL
Yu-shan Hu1, Jun-hua Liu, Xing-lin Pang
1Department of Pathogenic Biology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Objective:
To investigate the value of grOEL gene sequence in phylogenetic analysis and typing of Salmonella.
Methods:
The grOEL gene was amplified by PCR, sequenced and analyzed using Bioedit and DNAstar software. The Salmonella strains were identified using PCR-restriction fragment length polymophism (PCR-RFLP).
Results:
The conservative and variable regions of grOEL gene of Salmonella serogroup were separately distributed and most of the small mutant regions distributed intermittently among the conservative regions. The phylogenetic tree of Salmonella based on the nucleotides differed from that generated based on the amino acid sequence. O8, O9 and O10 had the closest consanguinity, and 5 patterns were identified by PCR-RFLP.
Conclusion:
The grOEL gene can be used as a genetic marker for phylogenetic analysis of Salmonella and also as a target sequence for Salmonella typing identification.
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