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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 19, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Absolute quantification of microRNAs by using a universal reference.

Ute Bissels1, Stefan Wild, Stefan Tomiuk

  • 1Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany.

RNA (New York, N.Y.)
|October 29, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microarray method for absolute quantification of microRNAs (miRNAs). The technique uses a universal reference to accurately measure miRNA levels in biological samples, overcoming limitations of previous methods.

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Last Updated: Jun 19, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Published on: May 16, 2012

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are small regulatory RNAs crucial for cellular processes.
  • Existing methods for miRNA identification and quantification face challenges due to their short length and high melting temperature variability.
  • Microarray technology offers parallel measurement but has limitations in absolute quantification.

Purpose of the Study:

  • To develop a microarray-based approach for global and absolute quantification of microRNAs.
  • To overcome the limitations of existing methods for accurate miRNA measurement.
  • To enable precise quantification of miRNA copy numbers in various biological samples.

Main Methods:

  • A novel microarray method employing parallel hybridization of labeled samples (Cy5) and a universal reference (Cy3) of synthetic miRNAs.
  • Utilizing a microarray slide with probes for human, mouse, rat, and viral miRNAs (miRBase 12.0).
  • Quantifying individual miRNAs against a universal reference to correct for sequence, labeling, and hybridization biases.

Main Results:

  • Demonstrated accuracy of the method through various spike-in experiments.
  • Successfully quantified miRNA copy numbers in liver samples.
  • Quantified miRNA copy numbers in CD34(+)/CD133(-) hematopoietic progenitor cells.

Conclusions:

  • The presented microarray approach enables accurate global and absolute quantification of microRNAs.
  • This method overcomes biases inherent in miRNA detection and quantification.
  • The technique is applicable for determining miRNA copy numbers in diverse biological contexts.