Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Lamellipodia Formation

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...

Propagation of Action Potentials

Propagation of Action Potentials

The propagation of an action potential refers to the process by which a nerve impulse, or "action potential," travels along a neuron.
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Localization and Performance of Auditory Brainstem Implants Based on MRI Measures of Paddle Placement.

Journal of neurological surgery. Part B, Skull base·2026

Same author

Apical Electrode Placement to Augment Intracochlear Current in Patients With an Ossified Cochlea and Incomplete Electrode Array Insertion.

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·2026

Same author

Airway molecular signatures in antibody-mediated lung transplant rejection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026

Same author

Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in <i>Drosophila</i> Follicle Stem Cells via Transcriptional Regulation.

bioRxiv : the preprint server for biology·2026

Same author

ARX mutation-associated interneuron defects provide insights into mechanisms underlying developmental epilepsies.

Brain : a journal of neurology·2026

Same author

Contrastive learning enhances fairness in pathology artificial intelligence systems.

Cell reports. Medicine·2025

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

Leading process branch instability in Lis1+/- nonradially migrating interneurons.

Pallavi P Gopal¹, Jacqueline C Simonet, William Shapiro

¹University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

Cerebral Cortex (New York, N.Y. : 1991)

|October 29, 2009

Summary

Lis1 protein is crucial for stabilizing new branches during mammalian forebrain neuron migration. Its role in the platelet-activating factor (PAF) pathway impacts microtubule stability and neuronal pathfinding.

More Related Videos

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Published on: April 21, 2011

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Published on: March 8, 2024

Related Experiment Videos

Last Updated: Jun 19, 2026

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Published on: April 21, 2011

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Published on: March 8, 2024

Area of Science:

Neuroscience
Developmental Biology
Cell Biology

Background:

Mammalian forebrain development involves complex neuronal migration guided by external cues.
The precise mechanisms by which migrating neurons sense and respond to guidance cues, particularly cytoskeletal regulation of branching, remain unclear.
Microtubule-associated protein Lis1 (Lis1) has been implicated in neuronal migration, with its deficiency linked to reduced branching.

Purpose of the Study:

To investigate the role of Lis1 in regulating branch stabilization during neuronal migration.
To elucidate the underlying cytoskeletal events and molecular pathways involved in Lis1-mediated branching.
To determine if Lis1's function in branching is linked to its role in the platelet-activating factor (PAF) pathway.

Main Methods:

Time-lapse imaging of migrating neurons from Lis1(+/-) and Lis1(+/+) medial ganglionic eminence explant cultures.
Analysis of microtubule acetylation as a marker for stabilization in leading processes.
Experimental manipulation using exogenous platelet-activating factor (PAF) and a PAF antagonist in wild-type and Lis1(+/-) neurons.

Main Results:

Lis1(+/-) neurons exhibit a defect in branch stabilization, not initiation, during migration.
Reduced expression of stabilized, acetylated microtubules was observed in the leading processes of Lis1(+/-) neurons.
Exogenous PAF mimicked the branch instability in wild-type neurons, while a PAF antagonist rescued branching in Lis1(+/-) neurons.

Conclusions:

Lis1 plays a critical role in stabilizing new leading process branches during neuronal migration.
Lis1 modulates branch stability and microtubule stabilization through the platelet-activating factor (PAF) pathway.
These findings reveal a novel mechanism for guidance cue response in migrating neurons, involving Lis1 and the PAF pathway.