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Aspergillus nidulans wetA activates spore-specific gene expression.

M A Marshall1, W E Timberlake

  • 1Departments of Genetics, University of Georgia, Athens 30602.

Molecular and Cellular Biology
|January 1, 1991
PubMed
Summary

The Aspergillus nidulans wetA gene is crucial for asexual spore maturation, controlling cell wall impermeability and dormancy. Its activation leads to the expression of late-stage sporulation genes, ensuring spore survival.

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Area of Science:

  • Molecular Biology
  • Mycology
  • Developmental Biology

Background:

  • The Aspergillus nidulans wetA gene is essential for asexual spore (conidia) maturation.
  • Mutations in wetA lead to permeable conidia that fail to mature and undergo autolysis.
  • wetA expression is regulated by the brlA and abaA genes during conidiogenesis.

Purpose of the Study:

  • To investigate whether wetA regulates the expression of other sporulation-specific genes.
  • To understand the role of wetA in the late stages of conidial development.

Main Methods:

  • A fusion construct of the wetA coding region with a nutritionally regulated promoter was created.
  • This construct allowed wetA activation in vegetative hyphae under conditions suppressing conidiation.

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  • Transcript accumulation was analyzed using Northern blotting.

    • Main Results:

    • Expression of wetA in vegetative hyphae inhibited growth and induced excessive branching.
    • wetA did not activate the upstream regulatory genes brlA or abaA.
    • wetA induced the accumulation of transcripts for genes normally expressed late in conidiation and stored in mature spores.
    • wetA also activated the wA gene, whose mRNA accumulates in phialide cells, suggesting a role beyond mature spores.

    Conclusions:

    • wetA directly or indirectly regulates the expression of specific genes involved in late-stage conidiation.
    • wetA plays a role in the synthesis or assembly of the final two conidial wall layers, conferring impermeability and dormancy.
    • wetA may also have regulatory functions in sporogenous phialide cells.