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Updated: Jun 19, 2026
Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
Published on: May 9, 2023
[Expression of M4 receptor in normal guinea pigs retina]
Yan Gao1, Jun-Wen Zeng, Gui-Xiang Liu
1The Affliated Hospital of Medical College of Qingdao University, Qingdao 266003, China. gaoyansd@yahoo.com.cn
Objective:
To detect the expression and distribution of M4 receptor in normal guinea pigs retina.
Methods:
Twenty one normal 4-week-old triad color guinea were selected. Eyes were enucleated. Seven eyes were fixed, paraffin-embedded and cut into sections. Expression and distribution of M4 receptor were detected using immunohisto chemically. The rest eyes were dissected. Tissue of retina were separated. RNA and protein of different tissue were extracted respectively. M4 receptor at mRNA level and protein level were detected by RT-PCR and Western blot methods.
Results:
receptor at mRNA level was detected in normal guinea retina, with the amplification product at 221 bp. No product was detected in negative control. M4 receptor at protein level was detected in normal guinea retina, with signal band at 38,000. No signal was detect in negative control. As detected by immunohistochemistry, M4 receptor was detected in RPE cells, photoreceptor layer, outer nuclear layer, inner nuclear layer, most ganglion cells and the junctional zone of inner plexiform layer and inner nuclear layer.
Conclusion:
M4 receptor was expressed in normal guinea retina.
