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Updated: Jun 19, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Müeller-Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus
Eidi Alvarado-Ramírez1, Josep M Torres-Rodríguez, Francisca Murciano
1Infectious Disease and Mycology Research Unit. Institut Municipal d 'Investigació Mèdica, Parc de Recerca Biomèdica de Barcelona, Barcelona, Spain. evictoriaalvarado@hotmail.com
Abstract:
Mueller-Hinton modified agar (MH-GMB) was compared with RPMI + 2% glucose-agar to determine the MICs of 80 isolates of Cryptococcus neoformans and C. gattii to posaconazole with Etest. MH-GMB minimised trailing and agreement between both media was 94%. Agreement of M27-A2 microbroth reference method was 98% with RPMI and 94% with MB-GMB.
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