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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Aptamers as a model for functional evaluation of LNA and 2'-amino LNA
Frank J Hernandez1, Neerja Kalra, Jesper Wengel
1Biochemistry and Molecular Biology Department, Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense M 5230, Denmark.
Abstract:
The affinity change upon incorporation of LNA and 2'-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2'-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.
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