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Related Experiment Video

Updated: Jun 19, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Fluorescence lifetime imaging in turbid media.

M A O'Leary, D A Boas, X D Li

|October 30, 2009

Summary

Fluorophore lifetime imaging can detect tissue conditions like oxygenation, but scattering in tissue complicates measurements. This study develops a method using diffuse photon density waves for accurate lifetime imaging in complex biological tissues.

Area of Science:

Biomedical Optics
Biophotonics
Medical Imaging

Background:

Fluorophore lifetime is sensitive to microenvironmental factors like oxygenation, pH, and glucose.
Optical properties of biological tissues, such as scattering and absorption, hinder accurate lifetime measurements.
Existing methods struggle with the complexity of optically thick, scattering biological tissues.

Purpose of the Study:

To develop a robust method for fluorescence lifetime tomography in highly scattering media.
To overcome the limitations of traditional lifetime measurements in complex biological environments.
To enable quantitative imaging of fluorophore distribution and lifetime in turbid tissues.

Main Methods:

Formulation of the inverse problem for fluorescence lifetime tomography.

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Last Updated: Jun 19, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: February 9, 2012

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

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Utilizing diffuse photon density waves to probe scattering media.

Employing simulated measurements in heterogeneous turbid media for validation.

Main Results:

Successful derivation of spatial images of heterogeneous fluorophore distribution.
Accurate reconstruction of spatially varying fluorescence lifetime values.
Demonstration of the technique's efficacy in simulated complex tissue models.

Conclusions:

The developed fluorescence lifetime tomography technique is effective for imaging in scattering tissues.
This method provides a pathway for non-invasive monitoring of physiological parameters.
The approach holds promise for advancing biomedical diagnostics and research.