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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jun 19, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Scanning plasmon optical microscope operation in atomic force microscope mode.

Y K Kim, J B Ketterson, D J Morgan

    Optics Letters
    |October 30, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new scanning plasmon optical microscope uses an atomic force microscope to create nanoscale optical images of dye-molecule crystals on silver films, offering high-resolution surface analysis.

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    Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

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    Related Experiment Videos

    Last Updated: Jun 19, 2026

    Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
    06:45

    Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

    Published on: February 28, 2019

    Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
    14:13

    Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

    Published on: October 24, 2014

    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
    09:20

    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

    Published on: August 11, 2020

    Area of Science:

    • Nanotechnology
    • Surface Science
    • Optical Microscopy

    Background:

    • Scanning plasmon optical microscopy (SPOM) typically uses scanning tunneling microscopy (STM).
    • Achieving nanoscale optical imaging requires advanced surface scanning techniques.
    • Dye-molecule crystals on conductive surfaces are of interest for optical applications.

    Purpose of the Study:

    • To investigate the feasibility of operating a scanning plasmon optical microscope with an atomic force microscope (AFM).
    • To demonstrate nanoscale optical imaging of dye-molecule crystals using this novel configuration.
    • To present high-resolution images of dye-molecule crystal islands.

    Main Methods:

    • Integration of an atomic force microscope (AFM) with a scanning plasmon optical microscope setup.
    • Surface scanning of dye-molecule crystals adsorbed on a silver film.
    • Acquisition and analysis of nanoscale optical images.

    Main Results:

    • Successful operation of a scanning plasmon optical microscope utilizing AFM.
    • Generation of nanoscale optical images of dye-molecule crystals.
    • Visualization of dye-molecule crystal islands with high resolution.

    Conclusions:

    • AFM can be effectively employed as the scanning probe for SPOM.
    • This integrated approach enables high-resolution nanoscale optical imaging of molecular adsorbates.
    • The presented technique offers a promising tool for surface analysis at the nanoscale.