Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional effects of replacing human alpha- and beta-globins with their embryonic globin homologues in defined haemoglobin heterotetramers.

British journal of haematology·2000

Same author

Characterization of Brachyury-downstream notochord genes in the Ciona intestinalis embryo.

Developmental biology·2000

Same author

Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk.

Nature·2000

Same author

Structure determination of [Arg8]vasopressin methylenedithioether in dimethylsulfoxide using NMR.

European journal of biochemistry·2000

Same author

Prediction of embolism in atrial fibrillation: classification of left atrial thrombi by transesophageal echocardiography.

Japanese circulation journal·2000

Same author

Primary hepatic carcinoid tumor: a case report.

Hepato-gastroenterology·2000

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Enhanced backscattering of partially coherent light.

T Okamoto, T Asakura

|October 30, 2009

Summary

Investigating light backscattering from random media with partially coherent light reveals that coherence suppresses the backscattering cone. This effect arises from breaking the time-reversal symmetry in multiple-scattering paths.

Area of Science:

Optics and Photonics
Wave Phenomena
Condensed Matter Physics

Background:

Light backscattering from random media exhibits enhanced intensity.
The influence of the spatial coherence of incident light on backscattering is not fully understood.
Existing theories often assume fully coherent or incoherent illumination.

Purpose of the Study:

To analyze the backscattering enhancement of light from random media under spatially partially coherent illumination.
To incorporate the effect of light coherence into existing backscattering theories.
To investigate the deformation and suppression of the backscattering cone due to coherence.

Main Methods:

Utilizing the angular correlation description for the illuminating light field to incorporate coherence.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Related Experiment Videos

Last Updated: Jun 19, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Applying an analogy to the van Cittert-Zernike theorem for infinite illumination areas.

Analyzing the breaking of time-reversal symmetry in multiple-scattering paths.

Main Results:

The intensity distribution of backscattered light is expressed as a sum of contributions from incident plane-wave components.
Partial coherence of the illuminating light leads to partial suppression and deformation of the backscattering cone.
Breaking of time-reversal symmetry in multiple-scattering paths is identified as the cause of these effects.

Conclusions:

Spatial coherence of incident light significantly modifies the backscattering enhancement from random media.
The backscattering cone is not a universal feature and can be altered by illumination conditions.
Understanding these effects is crucial for applications involving light propagation in disordered materials.