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Antioxidant and cytotoxic activities of Centella asiatica (L) Urb
Frederico Pittella1, Rafael C Dutra1, Dalton D Junior2
1Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil.
Abstract:
In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC(50) value of 31.25 microg/mL. The AE had a promising activity against mouse melanoma (B(16)F(1)), human breast cancer (MDA MB-231) and rat glioma (C(6)) cell lines, with IC(50) values of 698.0, 648.0 and 1000.0 microg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.
