Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Necrosis01:16

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Doctor and Patient in the Soviet Union.

Postgraduate medical journal·2011
Same author

Injuries and special concerns of female figure skaters.

Clinics in sports medicine·2000
Same author

Variation in the deoxynucleotide composition between organic and nonorganic strawberries.

Ecotoxicology and environmental safety·1999
Same author

Interstrain variation in the deoxynucleotide composition of Cryptococcus neoformans: nucleotide composition of Cryptococcus neoformans.

Medical mycology·1998
Same author

The use of fluoride in the UK's water supply has been shown to improve the health of children's teeth.

Nursing times·1998
Same author

Chronic ethanol feeding alters the structure and function of the epidermal growth factor receptor in rat stomach.

Alcohol (Fayetteville, N.Y.)·1996
Same journal

A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

The Journal of cell biology·2026
Same journal

Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

The Journal of cell biology·2026
Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026
Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
10:40

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Published on: January 26, 2018

IONIC EFFECTS ON LIGNIFICATION AND PEROXIDASE IN TISSUE CULTURES.

J Lipetz1, A J Garro

  • 1Laboratory of Plant Morphogenesis, Manhattan College, Bronx, New York.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

Specific ions like calcium and magnesium control peroxidase release from plant cell walls, influencing lignin deposition. This enzyme release is not from cell damage but ion concentration.

More Related Videos

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
10:39

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

Related Experiment Videos

Last Updated: Jun 19, 2026

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
10:40

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Published on: January 26, 2018

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
10:39

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

Area of Science:

  • Plant biochemistry
  • Cell biology
  • Biophysics

Background:

  • Crown gall tumor tissues release peroxidase into the medium.
  • Peroxidase release is influenced by specific ion concentrations.
  • This release is not attributed to diffusion from damaged cells.

Purpose of the Study:

  • To investigate the role of specific ions in controlling peroxidase release from plant cell cultures.
  • To understand the mechanism of ion-mediated enzyme release and its localization.
  • To establish a link between ion concentration, peroxidase levels, and lignin deposition.

Main Methods:

  • Utilized crown gall tumor tissue cultures.
  • Measured peroxidase release into the culture medium.
  • Performed cytochemical localization of peroxidase on cell walls and cytoplasm.
  • Isolated and washed cell wall fractions to assess bound peroxidase.
  • Treated cell wall fractions with divalent cations and ammonium to release bound enzyme.

Main Results:

  • Calcium, magnesium, and ammonium ions significantly increased peroxidase release, in that order.
  • Peroxidase was localized on cell walls and within the cytoplasm.
  • Washed cell wall fractions retained bound peroxidase.
  • Divalent cations (Ca(++), Sr(++), Ba(++)) and ammonium (NH(4)(+)) released bound peroxidase from cell walls, with specific effectiveness orders.
  • Ion-induced peroxidase release from cell walls was demonstrated.

Conclusions:

  • Specific ions directly control peroxidase release from plant cell walls.
  • Ion-mediated peroxidase release affects enzyme levels on cell walls.
  • This mechanism provides a basis for understanding how ions regulate lignin deposition in plant cell walls.