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Updated: Jun 19, 2026
Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
Published on: December 31, 2016
METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
1Saul R. Korey Department of Neurology, and the Departments of Neurological Surgery and Pathology (Neuropathology), Albert Einstein College of Medicine of Yeshiva University, New York.
Abstract:
No significant change was found in the electrolytes and lipids of the brain analyzed after glutaraldehyde fixation by perfusion of laboratory animals; such fixation also satisfactorily preserves neural tissues for electron microscopy. The brains of normal and tumor-bearing C3H mice, Wistar rats, and New Zealand rabbits were studied. Little difference was found in the dry weight and the content of sodium, potassium, total lipid and lipid fractions, and in the sulfate space (S(35)O(4)) between specimens from unperfused and perfused animals, whether normal or tumor-bearing. The results suggest the possibility of using selected regions of the nervous system, dissected after fixation, for chemical study and at the same time characterizing similar regions morphologically with the electron microscope.
