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Updated: Jun 19, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae
Published on: November 17, 2023
Isolation of nuclei from yeast
1Laboratory of Molecular Biology and the Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706.
Abstract:
A method for isolation of nuclei from Saccharomyces cervisiae in high yield is described. The DNA/protein ratio of the isolated nuclei is 10 times higher than that of whole cells. Examination of these nuclei in phase and electron microscopes has shown them to be round bodies having a double membrane, microtubules, and a dark crescent at one end. The optimum conditions for extraction and resolution of histones of these nuclei on acrylamide gels have been investigated. The nuclei have an active RNA polymerase (E.C. 2.7.7.6) and are able to synthesize RNA in vitro. They are also readily stainable with Giemsa's, Feulgen's, and acridine orange methods.
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