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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Published on: December 18, 2019
LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial
K E Kuettner1, R Eisenstein, L W Soble
1Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612.
Abstract:
THE LOCALIZATION OF CHICK EMBRYONIC LYSOZYME WAS DETERMINED BY TWO TECHNIQUES: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.
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