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Related Experiment Video

Updated: Jun 19, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial

K E Kuettner¹, R Eisenstein, L W Soble

¹Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612.

The Journal of Cell Biology

|October 30, 2009

Summary

Chick embryonic lysozyme is mainly located outside cells in cartilage tissue. Researchers confirmed its identity with egg white lysozyme using enzymatic and immunologic methods.

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Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

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Published on: February 24, 2026

Area of Science:

Biochemistry
Developmental Biology
Cell Biology

Background:

Lysozyme is a key enzyme involved in innate immunity and tissue remodeling.
Understanding lysozyme's localization is crucial for comprehending its function during embryonic development.
Chick embryonic cartilage is a relevant model for studying extracellular matrix components.

Purpose of the Study:

To determine the precise localization of lysozyme within chick embryonic cartilage.
To characterize the isolated cartilage lysozyme and compare it to known lysozyme forms.
To establish a method for isolating viable chondrocytes for further study.

Main Methods:

Enzymatic digestion to assess the rate of lysozyme release from tissue.
Immunocytochemistry to visualize lysozyme's location within the tissue.
Isolation and partial characterization of cartilage lysozyme.
Development of a chondrocyte isolation protocol.

Main Results:

Both enzymatic digestion and immunocytochemistry revealed that chick embryonic lysozyme is predominantly extracellular.
Isolated cartilage lysozyme exhibited identical immunologic and enzymatic properties to egg white lysozyme.
A reliable method for obtaining large quantities of viable chondrocytes was established.

Conclusions:

Chick embryonic cartilage lysozyme primarily resides in the extracellular space.
The findings suggest a conserved nature of lysozyme across different avian tissues.
The developed chondrocyte isolation technique facilitates future research on cartilage development and function.