Related Experiment Video
Updated: Jun 19, 2026
07:27
The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
Published on: October 21, 2017
EXPERIMENTAL LIVER NECROSIS. V. THE FATS AND LIPOIDS
1Bender Laboratory, Albany, N. Y.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Liver fat changes in dogs treated with haemotoxic serum are independent of necrosis. Fat accumulation results from fatty radical splitting, not protein breakdown, with altered fat composition observed.
Area of Science:
- Biochemistry
- Pathology
- Veterinary Medicine
Background:
- Haemotoxic serum can induce liver damage, including necrosis.
- Understanding the biochemical changes in liver tissue during such conditions is crucial.
Purpose of the Study:
- To investigate the relationship between liver fat content and necrosis in dogs treated with haemotoxic serum.
- To elucidate the biochemical mechanisms underlying fatty changes in the liver.
Main Methods:
- Chemical extraction of liver tissue to determine fat content and composition.
- Microchemical staining (Scharlach R) for fat visualization.
- Analysis of nitrogen and phosphorus content in extracted substances.
Main Results:
- Liver fat content changes were not correlated with the degree of necrosis.
- Increased tissue water content was linked to nitrogenous autolysis, not fat deposition.
- Fat appearance involved splitting of fatty radicals, with altered fat composition (lower iodine equivalent).
- Phosphorus to nitrogen ratios remained constant, indicating stable relationships between protagon, jecorin, and lecithins.
Conclusions:
- Liver fat accumulation in response to haemotoxic serum is a complex process not directly tied to necrosis severity.
- Biochemical analysis and microchemical staining provide consistent insights into these fatty changes.
Related Concept Videos
01:16Necrosis
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
01:23Cirrhosis I: Introduction
Cirrhosis is a chronic, irreversible liver disease characterized by the widespread replacement of healthy liver tissue with fibrotic scar tissue and the formation of regenerative nodules.Etiology of cirrhosisCirrhosis results from sustained liver injury that triggers progressive fibrosis and structural remodeling. The underlying causes are diverse, encompassing common and less frequent clinical conditions. Regardless of the origin, all causes lead to chronic inflammation, hepatocyte loss, and...