Related Experiment Video
Updated: Jun 19, 2026
10:35
Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Published on: December 26, 2017
STUDIES ON ANTIBODY FORMATION BY PERITONEAL EXUDATE CELLS IN VITRO
1Merck Institute for Therapeutic Research, West Point, Pennsylvania.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Rabbit peritoneal exudate cells, likely monocytes, produced hemagglutinating antibody to bovine gamma globulin (BGG) in vitro for weeks. Endotoxin significantly boosted antibody production in these cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Peritoneal exudate cells are crucial in immune responses.
- Monocytes play a key role in antibody production and immune regulation.
Purpose of the Study:
- To investigate the antibody-producing capacity of rabbit peritoneal exudate cells, specifically monocytes, in vitro.
- To determine the effect of antigen exposure and endotoxin on monocyte antibody production.
Main Methods:
- Rabbit peritoneal exudate cells were cultured in vitro.
- Antibody production was measured using a hemagglutination assay.
- Monocytes were isolated and exposed to antigens (BGG, EA) and endotoxin.
Main Results:
- Peritoneal exudate cells produced hemagglutinating antibody to BGG for up to 3 weeks.
- Isolated monocytes produced antibody to BGG and EA in vitro.
- Endotoxin significantly enhanced monocyte antibody production, mimicking hyperimmunization.
Conclusions:
- Monocytes are capable of sustained antibody production in vitro.
- Endotoxin potentiates monocyte antibody synthesis.
- Monocytes undergo morphological changes in culture but retain antibody-producing function.
Related Concept Videos
01:15Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...