Related Experiment Video
Updated: Jun 19, 2026

Granulocyte-dependent Autoantibody-induced Skin Blistering
Published on: October 12, 2012
IMMUNOHISTOLOGIC STUDIES ON ANTIGEN-ANTIBODY REACTIONS IN THE AVASCULAR CORNEA : I. REACTIONS IN RABBITS ACTIVELY
F G Germuth1, A E Maumenee, L B Senterfit
1Departments of Pathology and Ophthalmology, the Johns Hopkins University School of Medicine, Baltimore, and the Laboratory of Experimental Pathology, Charlotte Memorial Hospital, Charlotte, North Carolina.
Abstract:
The injection of antigen into the center of the avascular cornea of homologously sensitized animals induced a ring of opacification between the center of the cornea and the limbus. This ring of opacification was composed of a line of deeply eosinophilic amorphous material in a matrix of swollen collagen fibers, palisaded by polymorphonuclear leucocytes. By use of fluor-tagged antigen, it was shown that the line of damage in the cornea coincided with the precipitation of antigen presumably by antibody entering the cornea from the limbal vessels. With the passage of time, the antigen-antibody precipitates were removed, at least in part by phagocytosis, and the ring of opacification was replaced by ingrowing blood vessels surrounded by plasma cells. Treatment of sensitized animals with nitrogen mustard showed that antigen-antibody interaction could injure the corneal stroma in the relative absence of polymorphonuclear leucocytes.
Related Concept Videos
Immunocytochemistry and Immunohistochemistry
These...
Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Hypersensitivity Reactions: Immune-Complex Reactions
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Cross-reactivity
Allergic Drug Reactions
