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The vascular phase, also known as vasospasm, is the initial stage of hemostasis, crucial for preventing excessive bleeding when a blood vessel is injured. After a vessel is cut, nerves in the damaged area trigger pain and other sensory impulses. Simultaneously, the smooth muscles in the vessel wall contract, resulting in a vascular spasm. This contraction reduces the vessel's diameter at the injury site, slowing or stopping blood loss through the vessel wall. Vascular spasms typically last for...

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Related Experiment Video

Updated: Jun 19, 2026

An in vivo Assay to Test Blood Vessel Permeability

An in vivo Assay to Test Blood Vessel Permeability

Published on: March 16, 2013

INCREASED VASCULAR PERMEABILITY INDUCED BY HUMAN PLASMIN.

¹Department of Medicine, Western Reserve University School of Medicine, and University Hospitals, Cleveland.

The Journal of Experimental Medicine

|October 30, 2009

Summary

Plasminogen activation increases vascular permeability by producing kinins, a process inhibited by soybean trypsin inhibitor. This suggests plasmin

Area of Science:

Biochemistry
Physiology
Pharmacology

Background:

Vascular permeability is crucial for physiological processes.
The role of plasmin in modulating vascular permeability requires further elucidation.

Purpose of the Study:

To investigate the mechanism by which plasminogen activation affects vascular permeability.
To identify mediators involved in plasmin-induced permeability changes.

Main Methods:

In vivo experiments using guinea pig skin models.
Activation of plasminogen using streptokinase and urokinase.
Assays for vascular permeability and inhibition studies with soybean trypsin inhibitor and triprolidine.
Incubation of plasmin with plasma fractions rich in prokinin.

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Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

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Last Updated: Jun 19, 2026

An in vivo Assay to Test Blood Vessel Permeability

An in vivo Assay to Test Blood Vessel Permeability

Published on: March 16, 2013

High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility

High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility

Published on: August 16, 2024

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

Main Results:

Activated plasminogen significantly increased vascular permeability in guinea pigs.
This effect was dose-dependently inhibited by soybean trypsin inhibitor.
Triprolidine did not affect the permeability increase.
Plasmin incubation with prokinin-rich plasma fractions generated permeability-enhancing activity.

Conclusions:

Plasminogen activation enhances vascular permeability.
The mechanism involves the generation of kinins, likely mediated by plasmin.
Soybean trypsin inhibitor can block this effect, indicating a serine protease-dependent pathway.