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Updated: Jun 19, 2026

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Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
Published on: January 24, 2025
A METHOD FOR CULTIVATING TREPONEMA PALLIDUM IN FLUID MEDIA
1Laboratories of The Rockefeller Institute for Medical Research, New York.
The Journal of Experimental Medicine
|October 30, 2009
Abstract:
1. A method is described for obtaining pure cultures of Treponema pallidum and allied species of spirochaetae in fluid media. 2. The principle of the method is based upon the superposition of a favorable culture medium upon a less favorable one. 3. The method here described is not suited to the growth of spirochaetae when they are admixed with contaminating bacteria.

